7RTY
Crystal Structure of PsfC from Pseudomonas syringae PB-5123
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-08-08 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.12713 |
Spacegroup name | I 41 |
Unit cell lengths | 106.831, 106.831, 83.010 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.963 |
R-factor | 0.21848 |
Rwork | 0.216 |
R-free | 0.25691 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.920 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.548 | 1.970 |
High resolution limit [Å] | 1.960 | 1.963 |
Rmerge | 0.090 | 0.919 |
Rmeas | 0.096 | |
Number of reflections | 30430 | 233 |
<I/σ(I)> | 15.6 | 2.3 |
Completeness [%] | 91.2 | 68.9 |
Redundancy | 7.8 | 6 |
CC(1/2) | 0.997 | 0.752 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.17-0.20 M sodium fluoride, 18-20% PEG 3,350, 0.1 M bis-tris propane pH 8.5 |