7RTX

Actophorin grown in microgravity

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	39.810, 45.800, 69.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.660 - 1.650
R-factor	0.1902
Rwork	0.188
R-free	0.22330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ahq
RMSD bond length	0.012
RMSD bond angle	1.202
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.660	1.709
High resolution limit [Å]	1.650	1.650
Rmerge	0.077	0.875
Number of reflections	28851	1542
<I/σ(I)>	11.63
Completeness [%]	99.5
Redundancy	6.1
CC(1/2)	0.998	0.816

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	10 mg/mL protein, 0.1 M MOPS, 16% Polyethylene glycol (8,000)