7RT7
Crystal structure of the RhsP2 C-terminal toxin domain in complex with its immunity protein, RhsI2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 112.456, 112.456, 324.331 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 97.390 - 2.490 |
R-factor | 0.201 |
Rwork | 0.195 |
R-free | 0.23200 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.662 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 97.390 | 2.550 |
High resolution limit [Å] | 2.490 | 2.500 |
Number of reflections | 83716 | 4486 |
<I/σ(I)> | 8.5 | |
Completeness [%] | 99.8 | |
Redundancy | 9.3 | |
CC(1/2) | 0.994 | 0.574 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.1 | 296 | 1.05 M sodium citrate, 0.1 M CHES |