7RT7
Crystal structure of the RhsP2 C-terminal toxin domain in complex with its immunity protein, RhsI2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 112.456, 112.456, 324.331 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 97.390 - 2.490 |
| R-factor | 0.201 |
| Rwork | 0.195 |
| R-free | 0.23200 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.662 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.390 | 2.550 |
| High resolution limit [Å] | 2.490 | 2.500 |
| Number of reflections | 83716 | 4486 |
| <I/σ(I)> | 8.5 | |
| Completeness [%] | 99.8 | |
| Redundancy | 9.3 | |
| CC(1/2) | 0.994 | 0.574 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.1 | 296 | 1.05 M sodium citrate, 0.1 M CHES |
