7RSS

Kod-RI incorporating DNA, n+2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 2 21 21
Unit cell lengths	66.259, 112.000, 149.197
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.087 - 2.710
R-factor	0.1636
Rwork	0.159
R-free	0.22470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vu9
RMSD bond length	0.008
RMSD bond angle	0.938
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.030	2.807
High resolution limit [Å]	2.710	2.710
Rmerge	0.026	0.137
Rmeas	0.037	0.193
Rpim	0.026	0.137
Number of reflections	30746	3037
<I/σ(I)>	13.14	4.74
Completeness [%]	99.4	99.8
Redundancy	2	2
CC(1/2)	0.998	0.949

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	Sodium acetate, sodium citrate, PEG 4000