7RSR

Kod-RI incorporating PMT, n+2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2021-05-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 2 21 21
Unit cell lengths	65.780, 111.618, 149.026
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.556 - 1.980
R-factor	0.177
Rwork	0.176
R-free	0.21060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vu9
RMSD bond length	0.007
RMSD bond angle	0.890
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.107	2.051
High resolution limit [Å]	1.980	1.980
Rmerge	0.074	1.204
Rmeas	0.079	1.298
Rpim	0.030	0.479
Number of reflections	77125	7617
<I/σ(I)>	17.86
Completeness [%]	99.9	99.97
Redundancy	7.2	7.3
CC(1/2)	0.999	0.741

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	Sodium acetate, sodium citrate, PEG 4000