7RSF
Acetylornithine deacetylase from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-15 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 46.700, 70.994, 289.884 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.150 - 2.130 |
| R-factor | 0.1899 |
| Rwork | 0.188 |
| R-free | 0.23360 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.617 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.150 | 46.150 | 2.180 |
| High resolution limit [Å] | 2.130 | 5.810 | 2.130 |
| Rmerge | 0.120 | 0.044 | 1.013 |
| Rmeas | 0.133 | 0.050 | 1.187 |
| Rpim | 0.057 | 0.022 | 0.604 |
| Number of reflections | 54637 | 3003 | 2396 |
| <I/σ(I)> | 6.2 | 1.02 | |
| Completeness [%] | 98.8 | 98.4 | 90.5 |
| Redundancy | 4.9 | 4.8 | 3.1 |
| CC(1/2) | 0.986 | 0.998 | 0.513 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.1 M Bis-Tris Propane, 1 M ammonium citrate |
