7RRH
Crystal structure of fast switching R66M/M159T mutant of fluorescent protein Dronpa (Dronpa2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 186.629, 71.536, 108.271 |
Unit cell angles | 90.00, 110.12, 90.00 |
Refinement procedure
Resolution | 37.768 - 1.747 |
R-factor | 0.1777 |
Rwork | 0.176 |
R-free | 0.20220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4uts |
RMSD bond length | 0.008 |
RMSD bond angle | 1.328 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.13rc2_2986) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.770 | 1.813 |
High resolution limit [Å] | 1.747 | 1.747 |
Rmerge | 0.069 | 1.850 |
Rmeas | 0.071 | 1.920 |
Rpim | 0.019 | 0.530 |
Number of reflections | 132790 | 12922 |
<I/σ(I)> | 18.4 | 1.2 |
Completeness [%] | 98.2 | 96.4 |
Redundancy | 13.5 | 12.8 |
CC(1/2) | 1.000 | 0.714 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 292 | 0.1 M Tris-HCl pH 7.4, 0.1 M MgCl2, 18% PEG 3350 |