7RR4
Structure of Deep-Sea Phage NrS-1 Primase-Polymerase N300 in complex with magnesium and pyrophosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-01 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 119.340, 56.903, 48.564 |
| Unit cell angles | 90.00, 93.60, 90.00 |
Refinement procedure
| Resolution | 51.340 - 1.860 |
| R-factor | 0.2167 |
| Rwork | 0.216 |
| R-free | 0.23210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7rr3 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.781 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.340 | 1.930 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.096 | 1.175 |
| Number of reflections | 26979 | 2708 |
| <I/σ(I)> | 12.8 | |
| Completeness [%] | 98.3 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 298 | 800 mM sodium phosphate monobasic, 1200 mM potassium phosphate dibasic, and 100 mM sodium acetate (pH 4.5) |
