7RQH

Crystal Structure of carboxyl-terminal processing protease A mutant S302A, CtpA_S302A, of Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-16
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.07803
Spacegroup name	H 3
Unit cell lengths	189.650, 189.650, 131.105
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.030 - 3.200
R-factor	0.2422
Rwork	0.241
R-free	0.26540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7rpq
RMSD bond length	0.003
RMSD bond angle	0.739
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((1.19_4092))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	94.820	3.370
High resolution limit [Å]	3.200	3.200
Rmerge	0.089
Number of reflections	28884	4245
<I/σ(I)>	15.6
Completeness [%]	99.6	99.9
Redundancy	10.3	10.6
CC(1/2)	0.999	0.524

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	293	0.1 M sodium acetate, pH 4.0, and 0.6 M ammonium dihydrogen phosphate at a concentration of 33 mg/mL