7RPY

X25-2 domain of Sca5 from Ruminococcus bromii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2018-12-05
Detector	RAYONIX MX300-HS
Wavelength(s)	0.979
Spacegroup name	P 32 2 1
Unit cell lengths	100.820, 100.820, 87.930
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.460 - 1.670
R-factor	0.1823
Rwork	0.182
R-free	0.19630
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.350
Data reduction software	HKL-2000
Data scaling software	xia2
Phasing software	AutoSol
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.460	1.730
High resolution limit [Å]	1.670	1.670
Number of reflections	60154	5957
<I/σ(I)>	17.1
Completeness [%]	100.0	100
Redundancy	9.2	9
CC(1/2)	1.000	0.480

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	298	2M Ammonium sulfate, 0.1M NaAcetate pH=4.6