7RPQ
Crystal Structure of carboxyl-terminal processing protease A, CtpA, of Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-16 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97931 |
| Spacegroup name | H 3 |
| Unit cell lengths | 187.489, 187.489, 132.011 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.830 - 3.300 |
| R-factor | 0.2375 |
| Rwork | 0.236 |
| R-free | 0.26360 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.757 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.19_4092)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.750 | 3.480 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.167 | 0.912 |
| Number of reflections | 26048 | 3847 |
| <I/σ(I)> | 10.8 | 2.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.6 | 11.4 |
| CC(1/2) | 0.997 | 0.434 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | 0.1 M sodium acetate, pH 4.0, and 0.6 M ammonium dihydrogen phosphate at a concentration of 33 mg/mL |
