7RP0
Structural Snapshots of Intermediates in the Gating of a K+ Channel
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-01-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.000032 |
Spacegroup name | I 4 |
Unit cell lengths | 155.330, 155.330, 76.270 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.917 - 2.480 |
R-factor | 0.2444 |
Rwork | 0.241 |
R-free | 0.27090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1k4c |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.980 | 2.580 |
High resolution limit [Å] | 2.478 | 2.480 |
Number of reflections | 58647 | 3643 |
<I/σ(I)> | 13.2 | |
Completeness [%] | 99.9 | |
Redundancy | 7.4 | |
CC(1/2) | 0.990 | 0.790 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.25 | 293 | 50 mM MES, pH 6.25, 50 mM magnesium acetate, 27% PEG400 |