7ROY

The structure of the Fem1B:FNIP1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.194992
Spacegroup name	I 4 2 2
Unit cell lengths	164.475, 164.475, 465.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.330 - 2.900
R-factor	0.2075
Rwork	0.206
R-free	0.23040
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.439
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	CRANK2 (2.0.207)
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.330	2.970
High resolution limit [Å]	2.900	2.900
Rmeas	0.334	4.993
Rpim	0.064	0.979
Number of reflections	70955	4478
<I/σ(I)>	12.8	0.9
Completeness [%]	100.0	100
Redundancy	27.3	25.8
CC(1/2)	0.999	0.452

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	FEM1B-FNIP1 complex (20 mg/mL) was mixed in a 1:1 ratio with the reservoir solution containing 6% isopropanol, 150 mM NaCl, and 100 mM HEPES-NaOH pH 7.5