7ROM
Crystal structure of Saccharomyces cerevisiae NADH-cytochrome b5 reductase 1 (Cbr1) fragment (residues 28-284) bound to FAD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-18 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97903 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 72.225, 72.225, 114.669 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.650 - 1.650 |
R-factor | 0.1569 |
Rwork | 0.155 |
R-free | 0.18810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2eix |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.650 | 46.650 | 1.680 |
High resolution limit [Å] | 1.650 | 9.040 | 1.650 |
Rmerge | 0.052 | 0.032 | 0.576 |
Rmeas | 0.055 | 0.034 | 0.612 |
Rpim | 0.018 | 0.012 | 0.203 |
Total number of observations | 322326 | 1962 | 16216 |
Number of reflections | 36978 | 264 | 1816 |
<I/σ(I)> | 21.7 | 48.2 | 3.5 |
Completeness [%] | 99.6 | 96 | 100 |
Redundancy | 8.7 | 7.4 | 8.9 |
CC(1/2) | 0.999 | 0.999 | 0.870 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 295 | 20 % (w/v) PEG 3350 and 200 mM sodium thiocyanate |