7RMW
Crystal structure of B. subtilis PurR bound to ppGpp
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-12-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 60.264, 90.969, 98.708 |
Unit cell angles | 62.75, 75.36, 78.53 |
Refinement procedure
Resolution | 80.494 - 2.450 |
R-factor | 0.1957 |
Rwork | 0.195 |
R-free | 0.23970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1p4a |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.500 | 2.520 |
High resolution limit [Å] | 2.450 | 2.450 |
Rpim | 0.039 | 0.277 |
Number of reflections | 62863 | 4598 |
<I/σ(I)> | 7.8 | 2.3 |
Completeness [%] | 95.2 | |
Redundancy | 3 | |
CC(1/2) | 0.997 | 0.940 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.1 M MES pH 6.5, 18% PEG 1500 |