7RMM
Structure of N74D mutant of disulfide stabilized HIV-1 CA hexamer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2020-10-13 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 6 |
Unit cell lengths | 157.360, 157.360, 56.575 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.430 - 1.970 |
R-factor | 0.2192 |
Rwork | 0.218 |
R-free | 0.24650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6vkv |
RMSD bond length | 0.006 |
RMSD bond angle | 0.830 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.430 | 2.020 |
High resolution limit [Å] | 1.970 | 1.970 |
Rmerge | 0.106 | 1.308 |
Number of reflections | 56827 | 4002 |
<I/σ(I)> | 15.8 | 1.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.9 | 10.2 |
CC(1/2) | 0.999 | 0.639 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 0.2M NaI, 5% peg 3350, 6% glycerol, 0.1M sodium cacodylate trihydrate pH 6.5 |