7RM3

Antibody 2E10.E9 in complex with P. vivax CSP peptide ANGAGNQPGANGAGNQPGANGAGGQAA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-08-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.033167
Spacegroup name	P 1 21 1
Unit cell lengths	56.404, 144.436, 60.471
Unit cell angles	90.00, 102.83, 90.00

Refinement procedure

Resolution	29.480 - 2.680
R-factor	0.2081
Rwork	0.206
R-free	0.23940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uyw
RMSD bond length	0.009
RMSD bond angle	1.241
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.480	2.780
High resolution limit [Å]	2.680	2.680
Number of reflections	26439	2627
<I/σ(I)>	9.2	1.6
Completeness [%]	99.9	100
Redundancy	7	7.1
CC(1/2)	0.993	0.578

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.1 M sodium cacodylate pH 6.5, 0.2 M sodium acetate and 30% (w/v) PEG 8000