7RLZ

Antibody 2F2 in complex with P. vivax CSP peptide GDRAAGQPAGNGAGGQAA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-10-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.033167
Spacegroup name	C 1 2 1
Unit cell lengths	93.360, 60.490, 159.060
Unit cell angles	90.00, 101.22, 90.00

Refinement procedure

Resolution	29.690 - 2.270
R-factor	0.1938
Rwork	0.191
R-free	0.23870
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.707
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.690	2.320
High resolution limit [Å]	2.250	2.250
Number of reflections	41601	3874
<I/σ(I)>	9.4	1.5
Completeness [%]	99.9
Redundancy	11.6
CC(1/2)	0.927	0.435

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2 M di-sodium tartrate and 20% (w/v) PEG 3350