7RLZ
Antibody 2F2 in complex with P. vivax CSP peptide GDRAAGQPAGNGAGGQAA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2020-10-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.033167 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 93.360, 60.490, 159.060 |
Unit cell angles | 90.00, 101.22, 90.00 |
Refinement procedure
Resolution | 29.690 - 2.270 |
R-factor | 0.1938 |
Rwork | 0.191 |
R-free | 0.23870 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.707 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.690 | 2.320 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 41601 | 3874 |
<I/σ(I)> | 9.4 | 1.5 |
Completeness [%] | 99.9 | |
Redundancy | 11.6 | |
CC(1/2) | 0.927 | 0.435 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.2 M di-sodium tartrate and 20% (w/v) PEG 3350 |