7RLY
Antibody 2F2 in complex with P. vivax CSP peptide DRAAGQPAGDRADGQPA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2020-02-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.033167 |
Spacegroup name | P 1 |
Unit cell lengths | 71.723, 82.287, 82.747 |
Unit cell angles | 95.26, 113.78, 111.47 |
Refinement procedure
Resolution | 29.610 - 2.670 |
R-factor | 0.1875 |
Rwork | 0.185 |
R-free | 0.22720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xiw |
RMSD bond length | 0.002 |
RMSD bond angle | 0.570 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.610 | 2.770 |
High resolution limit [Å] | 2.670 | 2.670 |
Number of reflections | 43179 | 4542 |
<I/σ(I)> | 4.7 | 1.5 |
Completeness [%] | 98.3 | 97.8 |
Redundancy | 3.6 | 3.5 |
CC(1/2) | 0.987 | 0.669 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1M sodium citrate pH 5.0 and PEG 6000 |