7RLY

Antibody 2F2 in complex with P. vivax CSP peptide DRAAGQPAGDRADGQPA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-02-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.033167
Spacegroup name	P 1
Unit cell lengths	71.723, 82.287, 82.747
Unit cell angles	95.26, 113.78, 111.47

Refinement procedure

Resolution	29.610 - 2.670
R-factor	0.1875
Rwork	0.185
R-free	0.22720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xiw
RMSD bond length	0.002
RMSD bond angle	0.570
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.610	2.770
High resolution limit [Å]	2.670	2.670
Number of reflections	43179	4542
<I/σ(I)>	4.7	1.5
Completeness [%]	98.3	97.8
Redundancy	3.6	3.5
CC(1/2)	0.987	0.669

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.1M sodium citrate pH 5.0 and PEG 6000