7RLV
Antibody 2F2 in complex with P. vivax CSP peptide GDRADGQPAGDRADGQPA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2020-10-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.033167 |
Spacegroup name | P 1 |
Unit cell lengths | 71.487, 81.425, 82.263 |
Unit cell angles | 94.59, 114.10, 111.60 |
Refinement procedure
Resolution | 29.480 - 2.200 |
R-factor | 0.1814 |
Rwork | 0.179 |
R-free | 0.22020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xiw |
RMSD bond length | 0.007 |
RMSD bond angle | 0.867 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.480 | 2.250 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 75088 | 4440 |
<I/σ(I)> | 5.2 | 1.5 |
Completeness [%] | 97.5 | 97.1 |
Redundancy | 3.5 | 3.1 |
CC(1/2) | 0.965 | 0.527 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.2 M di-sodium tartrate and 20% (w/v) PEG 3350 |