7RLM
Crystal Structure of Potato Leafroll Virus (PLRV) Coat Protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-12 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 |
Unit cell lengths | 36.863, 67.479, 75.887 |
Unit cell angles | 79.67, 76.22, 81.67 |
Refinement procedure
Resolution | 72.860 - 1.800 |
R-factor | 0.1698 |
Rwork | 0.167 |
R-free | 0.21750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6sco |
RMSD bond length | 0.007 |
RMSD bond angle | 0.886 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 72.860 | 1.558 |
High resolution limit [Å] | 1.504 | 1.504 |
Rmerge | 0.072 | |
Number of reflections | 177750 | 6323 |
<I/σ(I)> | 8.6 | |
Completeness [%] | 89.1 | |
Redundancy | 3.3 | |
CC(1/2) | 0.997 | 0.315 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1 M Bis-Tris propane, pH 7.7, 26% PEG3350, 0.1 M sodium bromide |