7RLL

Crystal structure of ARF3 from Candida albicans in complex with guanosine-3'-monophosphate-5'-diphosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	50.958, 75.852, 97.518
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.760 - 1.900
R-factor	0.188
Rwork	0.185
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1e0s
RMSD bond length	0.007
RMSD bond angle	1.153
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.110	1.326
Rpim	0.032	0.428
Number of reflections	30467	1471
<I/σ(I)>	34.2	1.8
Completeness [%]	99.9	100
Redundancy	12.4
CC(1/2)	0.999	0.921

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	0.1 M potassium thiocyanate, 30% (w/v) PEG 2K MME, 2 mM magnesium chloride, 1.5 mM GMP-PCP