7RJO

Crystal structure of human Bromodomain containing protein 4 (BRD4) in complex with hnRNPK

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.92
Spacegroup name	P 21 21 21
Unit cell lengths	48.454, 52.080, 53.325
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.230 - 1.380
R-factor	0.1771
Rwork	0.176
R-free	0.19790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3mxf
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (dev_3940)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	24.230	24.230	1.410
High resolution limit [Å]	1.380	7.580	1.380
Rmerge	0.054	0.053	0.683
Rmeas	0.057	0.056	0.736
Rpim	0.018	0.020	0.263
Total number of observations	263623	1581	9099
Number of reflections	27852	207	1275
<I/σ(I)>	22.3	44.8	2.4
Completeness [%]	98.9	96.1	92.4
Redundancy	9.5	7.6	7.1
CC(1/2)	0.999	0.996	0.907

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	292	25% w/v PEG3350, 0.2 M ammonium acetate, 0.1 M Bis-Tris