7RJO
Crystal structure of human Bromodomain containing protein 4 (BRD4) in complex with hnRNPK
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-09-16 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.92 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.454, 52.080, 53.325 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.230 - 1.380 |
R-factor | 0.1771 |
Rwork | 0.176 |
R-free | 0.19790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mxf |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (dev_3940) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 24.230 | 24.230 | 1.410 |
High resolution limit [Å] | 1.380 | 7.580 | 1.380 |
Rmerge | 0.054 | 0.053 | 0.683 |
Rmeas | 0.057 | 0.056 | 0.736 |
Rpim | 0.018 | 0.020 | 0.263 |
Total number of observations | 263623 | 1581 | 9099 |
Number of reflections | 27852 | 207 | 1275 |
<I/σ(I)> | 22.3 | 44.8 | 2.4 |
Completeness [%] | 98.9 | 96.1 | 92.4 |
Redundancy | 9.5 | 7.6 | 7.1 |
CC(1/2) | 0.999 | 0.996 | 0.907 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 292 | 25% w/v PEG3350, 0.2 M ammonium acetate, 0.1 M Bis-Tris |