7RJM
Crystal structure of human Bromodomain containing protein 3 (BRD3) in complex with ILF3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 59.009, 59.009, 133.966 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 23.870 - 2.100 |
R-factor | 0.194 |
Rwork | 0.192 |
R-free | 0.23720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6qju |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.22) |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 51.103 | 24.617 | 2.210 |
High resolution limit [Å] | 2.100 | 6.640 | 2.100 |
Rmerge | 0.039 | 0.575 | |
Rmeas | 0.064 | 0.041 | 0.607 |
Rpim | 0.021 | 0.015 | 0.192 |
Total number of observations | 155473 | 4714 | 23218 |
Number of reflections | 16445 | 589 | 2343 |
<I/σ(I)> | 19.5 | 42.4 | 4.1 |
Completeness [%] | 99.9 | 98 | 100 |
Redundancy | 9.5 | 8 | 9.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 292 | 25% w/v PEG3350, 0.1 M Bis-Tris, 0.2 M sodium chloride |