7RJ3
Crystal Structure of the Forkhead Associated (FHA) Domain of the Glycogen Accumulation Regulator (GarA) from Mycobacterium tuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-10-17 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.135, 56.755, 92.264 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.130 - 1.680 |
R-factor | 0.1769 |
Rwork | 0.174 |
R-free | 0.22280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6i2p |
RMSD bond length | 0.006 |
RMSD bond angle | 1.395 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.710 |
High resolution limit [Å] | 1.680 | 1.680 |
Rmerge | 0.065 | 0.789 |
Rmeas | 0.070 | 0.854 |
Rpim | 0.027 | 0.325 |
Number of reflections | 30373 | 1511 |
<I/σ(I)> | 26.9 | 2.61 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.8 | 6.8 |
CC(1/2) | 0.806 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.3 | 292 | 15.3 mg/mL protein in 0.5 M sodium chloride, 0.01 M Tris, pH 8.3 against JCSG+ screen G10 (0.15 M potassium bromide, 30% w/v PEG2000 MME) |