7RIQ

RNA polymerase II elongation complex scaffold 1 without polyamide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-10-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	167.849, 223.467, 193.032
Unit cell angles	90.00, 100.30, 90.00

Refinement procedure

Resolution	48.152 - 3.000
R-factor	0.2139
Rwork	0.213
R-free	0.26440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7rim
Data reduction software	XDS
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER
Refinement software	PHENIX (1.13)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.270	49.270	3.010
High resolution limit [Å]	2.960	16.210	2.960
Rmerge	0.309	0.084	4.687
Rmeas	0.331	0.091	5.025
Rpim	0.120	0.035	1.807
Total number of observations	1103679	5650	55348
Number of reflections	145165	876	7181
<I/σ(I)>	5.4	19.2	0.4
Completeness [%]	99.9	95.4	99.7
Redundancy	7.6	6.4	7.7
CC(1/2)	0.993	0.991	0.272

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 5mM dioxane, 5mM DTT, 10-12% PEG6000