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7RIP

RNA polymerase II elongation complex with hairpin polyamide Py-Im 1, scaffold 1 soaked with CTP

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsALS BEAMLINE 8.2.2
Synchrotron siteALS
Beamline8.2.2
Temperature [K]100
Detector technologyCCD
Collection date2019-08-23
DetectorADSC QUANTUM 315r
Wavelength(s)1.0
Spacegroup nameC 1 2 1
Unit cell lengths168.158, 223.041, 193.464
Unit cell angles90.00, 100.67, 90.00
Refinement procedure
Resolution49.225 - 3.300
R-factor0.2198
Rwork0.219
R-free0.26070
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)7rim
Data reduction softwareXDS
Data scaling softwareAimless (0.7.3)
Phasing softwarePHASER
Refinement softwarePHENIX (1.13)
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]49.23049.2303.330
High resolution limit [Å]3.27017.9103.270
Rmerge0.3800.0314.013
Rmeas0.4080.0344.302
Rpim0.1470.0121.546
Total number of observations829562479941475
Number of reflections1079236585363
<I/σ(I)>6.751.90.6
Completeness [%]100.095.3100
Redundancy7.77.37.7
CC(1/2)0.9880.9990.239
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP298390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 5mM dioxane, 5mM DTT, 10-12% PEG6000

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