7RIP
RNA polymerase II elongation complex with hairpin polyamide Py-Im 1, scaffold 1 soaked with CTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-08-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 168.158, 223.041, 193.464 |
Unit cell angles | 90.00, 100.67, 90.00 |
Refinement procedure
Resolution | 49.225 - 3.300 |
R-factor | 0.2198 |
Rwork | 0.219 |
R-free | 0.26070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7rim |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.3) |
Phasing software | PHASER |
Refinement software | PHENIX (1.13) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.230 | 49.230 | 3.330 |
High resolution limit [Å] | 3.270 | 17.910 | 3.270 |
Rmerge | 0.380 | 0.031 | 4.013 |
Rmeas | 0.408 | 0.034 | 4.302 |
Rpim | 0.147 | 0.012 | 1.546 |
Total number of observations | 829562 | 4799 | 41475 |
Number of reflections | 107923 | 658 | 5363 |
<I/σ(I)> | 6.7 | 51.9 | 0.6 |
Completeness [%] | 100.0 | 95.3 | 100 |
Redundancy | 7.7 | 7.3 | 7.7 |
CC(1/2) | 0.988 | 0.999 | 0.239 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 5mM dioxane, 5mM DTT, 10-12% PEG6000 |