7RIM
RNA polymerase II elongation complex with hairpin polyamide Py-Im 1, scaffold 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-06-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9999 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 167.220, 221.930, 193.710 |
Unit cell angles | 90.00, 99.63, 90.00 |
Refinement procedure
Resolution | 82.432 - 2.900 |
R-factor | 0.217 |
Rwork | 0.215 |
R-free | 0.25080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6uq2 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.13) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 82.432 |
High resolution limit [Å] | 2.900 |
Number of reflections | 153630 |
<I/σ(I)> | 5.83 |
Completeness [%] | 99.7 |
Redundancy | 2 |
CC(1/2) | 0.990 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 5mM dioxane, 5mM DTT, 10-12% PEG6000 |