7RID
Griffithsin variant Y28A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU R-AXIS IV |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2018-06-03 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 79.113, 79.113, 119.814 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.310 - 2.300 |
| R-factor | 0.1604 |
| Rwork | 0.152 |
| R-free | 0.16390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2guc |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.720 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.17.1) |
| Refinement software | PHENIX (1.17.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.096 | 0.282 |
| Number of reflections | 10413 | 511 |
| <I/σ(I)> | 30.5 | 8.1 |
| Completeness [%] | 99.9 | |
| Redundancy | 18.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M bis-tris, pH 6.5 0.2 M calcium chloride dihydrate 45% v/v (+/-)-2-methyl-2,4-pentanediol |
