7RIC
Griffithsin variant Y28W/Y68W/Y110W
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU R-AXIS IV |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2018-06-01 |
Detector | RIGAKU SATURN A200 |
Wavelength(s) | 1.54 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 71.162, 71.162, 71.488 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.220 - 1.950 |
R-factor | 0.1607 |
Rwork | 0.158 |
R-free | 0.18940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2guc |
RMSD bond length | 0.019 |
RMSD bond angle | 1.548 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.17.1) |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.980 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.044 | 0.101 |
Number of reflections | 15647 | 671 |
<I/σ(I)> | 49 | 11.5 |
Completeness [%] | 99.2 | 85.4 |
Redundancy | 10.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M bis-tris buffer, pH 6.5 0.1 M magnesium sulfate 3 M sodium chloride |