7RHW

Aspergillus fumigatus Enolase Bound to 2-Phosphoglycerate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2020-06-24
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	67.379, 83.290, 84.161
Unit cell angles	90.00, 97.45, 90.00

Refinement procedure

Resolution	41.725 - 1.900
R-factor	0.1916
Rwork	0.190
R-free	0.22720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7rhv
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.16-3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.320	42.320	1.940
High resolution limit [Å]	1.900	9.310	1.900
Rmerge	0.077	0.022	0.854
Rmeas	0.083	0.024	0.923
Rpim	0.032	0.009	0.347
Total number of observations	490423	4486	31412
Number of reflections	72696	650	4504
<I/σ(I)>	13.5	52.6	2.2
Completeness [%]	99.9	98.8	100
Redundancy	6.7	6.9	7
CC(1/2)	0.999	0.999	0.729

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	23% PEG 3350, 0.1 M Bis-Tris pH 5.5