7RHV

Aspergillus fumigatus Enolase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-09-30
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.954
Spacegroup name	P 1 21 1
Unit cell lengths	67.174, 83.969, 84.661
Unit cell angles	90.00, 97.56, 90.00

Refinement procedure

Resolution	42.390 - 2.000
R-factor	0.1629
Rwork	0.160
R-free	0.21360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1one
Data reduction software	XDS (20190315)
Data scaling software	Aimless (0.7.2)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.16-3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.393	42.390	2.050
High resolution limit [Å]	2.000	9.170	2.000
Rmerge	0.128	0.070	0.412
Rmeas	0.138	0.076	0.444
Rpim	0.051	0.029	0.163
Total number of observations		4743	32081
Number of reflections	63071	691	4414
<I/σ(I)>	9.8	19.1	3.4
Completeness [%]	100.0	98.8	100
Redundancy	7.2	6.9	7.3
CC(1/2)	0.995	0.995	0.957

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	23% PEG 3350, 0.15 M Bis-Tris pH 5.5