7RHP
Crystal Structure of Honeybee (Apis mellifera) glutathione S-transferase AmGSTD1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-07-09 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 60.090, 60.090, 232.050 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 52.040 - 1.540 |
R-factor | 0.1907 |
Rwork | 0.190 |
R-free | 0.20260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3wyw |
RMSD bond length | 0.006 |
RMSD bond angle | 0.879 |
Data reduction software | DIALS |
Data scaling software | xia2 |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.18.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.040 | 1.595 |
High resolution limit [Å] | 1.540 | 1.540 |
Rmerge | 0.078 | 0.973 |
Rmeas | 0.080 | |
Rpim | 0.019 | 0.285 |
Number of reflections | 37014 | 2976 |
<I/σ(I)> | 14.34 | 0.34 |
Completeness [%] | 95.0 | 73.91 |
Redundancy | 16.9 | 11.7 |
CC(1/2) | 1.000 | 0.772 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 291 | 0.2 M Sodium chloride, 20% w/v Polyethylene glycol 3350 |