7RHB

A new fluorescent protein darkmRuby at pH 8.0

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	108.087, 77.268, 66.808
Unit cell angles	90.00, 92.11, 90.00

Refinement procedure

Resolution	38.630 - 2.510
R-factor	0.229
Rwork	0.227
R-free	0.26640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3u0m
RMSD bond length	0.007
RMSD bond angle	1.580
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.630	2.610
High resolution limit [Å]	2.510	2.510
Number of reflections	18477	2069
<I/σ(I)>	6.1
Completeness [%]	98.0
Redundancy	3.11
CC(1/2)	0.995	0.822

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		285	1 M NaH2PO4 / K2HPO4