7RGV

Structure of Caulobacter crescentus Suppressor of copper sensitivity protein C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9536
Spacegroup name	P 63
Unit cell lengths	113.948, 113.948, 48.695
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.340 - 2.630
R-factor	0.2263
Rwork	0.224
R-free	0.25020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4VXW res 50-224 chain A
RMSD bond length	0.002
RMSD bond angle	0.446
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.340	2.740
High resolution limit [Å]	2.630	2.630
Rmerge	0.052	1.460
Rmeas	0.054	1.520
Rpim	0.016	0.431
Number of reflections	10854	1045
<I/σ(I)>	22.51	1.85
Completeness [%]	98.8	94.76
Redundancy	12	12.3
CC(1/2)	1.000	0.961

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.25	277.15	Protein reduced with DTT and purified in 25 mM HEPES pH 7.5, 150 mM NaCl. Protein concentrated to 130 mg/ml. 1ul of protein solution mixed with 1ul of crystallant solution: 39% MPD, 200 mM NaAcetate, 20 mM CaCl2 pH 7.25