7RGC

Crystal structure of triosephosphate isomerase from Candidatus Absconditabacteria (Sr1) bacterium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2021-02-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.95372
Spacegroup name	H 3 2
Unit cell lengths	151.797, 151.797, 118.439
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.000 - 2.090
R-factor	0.2397
Rwork	0.236
R-free	0.28910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1b9b
RMSD bond length	0.007
RMSD bond angle	0.894
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.820	2.220
High resolution limit [Å]	2.090	2.090
Number of reflections	30881	4732
<I/σ(I)>	12.74
Completeness [%]	99.2
Redundancy	20.3
CC(1/2)	0.999	0.830

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	2.3 M DL-Malic acid pH 7.0