7RG6

Importin alpha2 in complex with ORF4B Bat coronavirus HKU5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-03-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	78.692, 90.310, 100.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.660 - 2.100
R-factor	0.2058
Rwork	0.205
R-free	0.22280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bvt
RMSD bond length	0.002
RMSD bond angle	0.480
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19rc4_4035)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.990	2.270
High resolution limit [Å]	2.100	2.200
Rmerge	0.084	1.160
Rpim	0.059	0.831
Number of reflections	42118	4172
<I/σ(I)>	12.8	2.3
Completeness [%]	99.9
Redundancy	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	0.1M HEPES pH6.5, 0.6M Citrate, 0.01M DTT