7RG4
Importin alpha2 in complex with p50 NLS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2017-11-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.015, 89.748, 97.204 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.920 - 2.600 |
| R-factor | 0.218 |
| Rwork | 0.216 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6bvt |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.473 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19rc4_4035) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.920 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.127 | 1.321 |
| Rpim | 0.059 | 0.630 |
| Number of reflections | 21620 | 2587 |
| <I/σ(I)> | 10.8 | 2.1 |
| Completeness [%] | 99.9 | |
| Redundancy | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 296 | 0.7 M sodium citrate, 0.01M DTT, 0.1M sodium HEPES pH 7 |
