7RG2
Importin alpha2 in complex with MERS ORF4B R33A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2020-12-06 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9537 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.850, 89.870, 100.460 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.640 - 2.000 |
R-factor | 0.1998 |
Rwork | 0.199 |
R-free | 0.21630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6bvt |
RMSD bond length | 0.002 |
RMSD bond angle | 0.500 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19rc4_4035) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.960 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.051 | 0.640 |
Rpim | 0.040 | 0.500 |
Number of reflections | 48821 | 3553 |
<I/σ(I)> | 13.8 | 2 |
Completeness [%] | 99.8 | |
Redundancy | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 296 | 0.7 M Sodium Citrate, 0.1 M HEPES pH 6.5 |