7RFY
Importin alpha3 in complex with MERS ORF4B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2020-08-06 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9537 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.222, 59.711, 82.640 |
Unit cell angles | 90.00, 98.89, 90.00 |
Refinement procedure
Resolution | 25.990 - 2.500 |
R-factor | 0.2497 |
Rwork | 0.249 |
R-free | 0.26730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6bvz |
RMSD bond length | 0.003 |
RMSD bond angle | 0.595 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | autoBUSTER (1.18.2_3874) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.990 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.110 | 0.870 |
Number of reflections | 15900 | 1762 |
<I/σ(I)> | 5.4 | 1.2 |
Completeness [%] | 98.2 | |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 296 | 0.2 M magnesium chloride, 0.1 M Tris pH 8.0, 20% w/v PEG 6000 |