7RFT
Domain 1 of Starch adherence system protein 20 (Sas20) from Ruminococcus bromii with maltotriose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-08-01 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 121.837, 64.668, 64.734 |
| Unit cell angles | 90.00, 102.76, 90.00 |
Refinement procedure
| Resolution | 29.520 - 1.530 |
| R-factor | 0.1761 |
| Rwork | 0.175 |
| R-free | 0.19710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7raw |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.203 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.560 |
| High resolution limit [Å] | 1.530 | 4.150 | 1.530 |
| Rmerge | 0.047 | 0.023 | 1.444 |
| Rmeas | 0.053 | 0.026 | 1.671 |
| Rpim | 0.025 | 0.012 | 0.828 |
| Total number of observations | 339801 | ||
| Number of reflections | 74182 | 3807 | 3699 |
| <I/σ(I)> | 10.7 | ||
| Completeness [%] | 100.0 | 99.6 | 99.9 |
| Redundancy | 4.6 | 4.5 | 4 |
| CC(1/2) | 0.999 | 0.364 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 27% PEG 4000, 0.2M MgCl, 0.1M Tris |
