7RFQ
STRUCTURE OF BACTERIAL SYLF DOMAIN CONTAINING PROTEIN, BETA CELL EXPANSION FACTOR A (BEFA)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2016-07-16 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.0, 0.9795 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 94.670, 64.550, 42.630 |
Unit cell angles | 90.00, 113.26, 90.00 |
Refinement procedure
Resolution | 37.350 - 1.270 |
R-factor | 0.132 |
Rwork | 0.131 |
R-free | 0.15500 |
Structure solution method | SIRAS |
Data reduction software | HKL-2000 |
Data scaling software | SCALA (3.3.22) |
Phasing software | AutoSol |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.350 | 1.320 |
High resolution limit [Å] | 1.270 | 1.270 |
Rmerge | 0.114 | |
Rmeas | 0.177 | 0.119 |
Rpim | 0.054 | 0.035 |
Total number of observations | 621902 | 23389 |
Number of reflections | 62064 | 2032 |
<I/σ(I)> | 8.7 | 17.2 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 10 | 11.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 295 | 25 % PEG 3350, 100 mM CITRIC ACID PH 3.5, 1 mM TCEP, 39 mM O-PHOSPHO-L-SERINE OR 39 mM SERINE, VAPOR |