7RFK

CamA Adenine Methyltransferase Complexed to Cognate Substrate DNA and Inhibitor Sinefungin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	82.155, 160.446, 231.304
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.290 - 2.050
R-factor	0.1893
Rwork	0.189
R-free	0.22500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7lt5
RMSD bond length	0.005
RMSD bond angle	0.732
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.290	2.120
High resolution limit [Å]	2.050	2.050
Rmerge	0.279	1.750
Rpim	0.067	0.676
Number of reflections	177994	15920
<I/σ(I)>	8.7	1.1
Completeness [%]	93.9	84.9
Redundancy	14.6	6.5
CC(1/2)		0.395

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	21~24% (w/v) polyethylene glycol 3350, 0.1 M Tris-HCl pH 7.0~7.5, 0.28 M potassium citrate