7RF9
O2-, PLP-dependent desaturase Plu4 intermediate-bound enzyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.922, 70.279, 138.568 |
Unit cell angles | 90.00, 98.11, 90.00 |
Refinement procedure
Resolution | 38.328 - 1.926 |
R-factor | 0.1532 |
Rwork | 0.151 |
R-free | 0.19430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6c3d |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.1) |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.160 | 39.160 | 1.960 |
High resolution limit [Å] | 1.926 | 10.550 | 1.930 |
Rmerge | 0.192 | 0.096 | 2.211 |
Rmeas | 0.199 | 0.099 | 2.307 |
Rpim | 0.054 | 0.026 | 0.646 |
Total number of observations | 9943 | 58931 | |
Number of reflections | 106646 | 706 | 4801 |
<I/σ(I)> | 11.1 | 29 | 1.9 |
Completeness [%] | 98.8 | 98.3 | 90.7 |
Redundancy | 13.6 | 14.1 | 12.3 |
CC(1/2) | 0.996 | 0.996 | 0.852 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Bis-Tris:HCl; 27% PEG 3350; 0.2 M ammonium acetate; microseeded from crystals grown at pH 6.0; soaked with 2 mM PLP and 3 mM L-arginine for 8 min |