7RF0
MYCOBACTERIUM ABSCESSUS TRNA METHYLTRANSFERASE IN COMPLEX WITH S-ADENOSYL-L-HOMOCYSTEINE AND MAGNESIUM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-02 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9802 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 74.398, 78.835, 86.656 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.220 - 1.590 |
R-factor | 0.1819 |
Rwork | 0.180 |
R-free | 0.21340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6nvr |
RMSD bond length | 0.006 |
RMSD bond angle | 0.980 |
Data reduction software | ADDREF |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.240 | 1.600 |
High resolution limit [Å] | 1.560 | 1.560 |
Number of reflections | 68820 | 4995 |
<I/σ(I)> | 18.8 | 1.9 |
Completeness [%] | 99.2 | |
Redundancy | 6.6 | |
CC(1/2) | 0.999 | 0.781 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Drops were 2ul of protein 24 mg/ml and 2ul buffer (0.1M sodium cacodylate and 2M ammonium nitrate pH 6.5); co-crystallized with SAH in a 1:1 ratio at a final concentration of 5mM SAH in the drops; and 4ul MgSO4 at 10mM were added to the drops before cryo-cooling |