7RE8

Class I MHC (HLA-A*02) presenting alpha fetoprotein peptide (AFP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-23
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	63.207, 87.310, 78.724
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.470 - 2.820
R-factor	0.191
Rwork	0.185
R-free	0.23900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5e00
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.480	2.870
High resolution limit [Å]	2.820	2.820
Number of reflections	20564	1016
<I/σ(I)>	8.4
Completeness [%]	99.0
Redundancy	6.5
CC(1/2)	0.991	0.918

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277.15	25 mM MES pH6.5, 150 mM NaCl, 25% v/v PEG 3350