7RE6
Crystal Structure of the brown dog tick (Rhipicephalus sanguineus) Arginine Kinase in absence of substrate or ligands
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-21 |
| Detector | DECTRIS EIGER R 1M |
| Wavelength(s) | 1.19 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.412, 61.410, 61.342 |
| Unit cell angles | 90.00, 96.56, 90.00 |
Refinement procedure
| Resolution | 35.820 - 1.530 |
| R-factor | 0.1567 |
| Rwork | 0.155 |
| R-free | 0.18570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4am1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.156 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.820 | 1.580 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Number of reflections | 52892 | 52890 |
| <I/σ(I)> | 8.38 | |
| Completeness [%] | 98.0 | 93.01 |
| Redundancy | 1.9 | |
| CC(1/2) | 0.887 | 0.887 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.8 | 289 | 0.1 M bis-tris pH 6.5, 25% p/v PEG 3350 |
