7RE5

Crystal Structure of The Tetramerization Domain (29-147) From Human Voltage-gated Potassium Channel Kv2.1 in P 41 21 2 Space Group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2021-05-05
Detector	RDI CMOS_8M
Wavelength(s)	1.07216
Spacegroup name	P 41 21 2
Unit cell lengths	78.754, 78.754, 214.803
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.370 - 2.500
R-factor	0.2273
Rwork	0.225
R-free	0.26750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kvt
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.370	44.370	2.600
High resolution limit [Å]	2.500	9.010	2.500
Rmerge	0.097	0.031	1.108
Rmeas	0.100	0.032	1.153
Rpim	0.027	0.009	0.316
Total number of observations		7398	35331
Number of reflections	44658	634	2696
<I/σ(I)>	26.4	77.5	2.5
Completeness [%]	100.0	99.4	100
Redundancy	13.7	11.7	13.1
CC(1/2)	0.999	1.000	0.837

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	0.2 M magnesium chloride hexahydrate, 0.1 M Tris, pH 8.5, 3.4 M 1,6-Hexanediol