7RDS
Structure of human NTHL1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-07-01 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.0332 |
Spacegroup name | P 63 |
Unit cell lengths | 124.800, 124.800, 42.250 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.850 - 2.500 |
R-factor | 0.202 |
Rwork | 0.200 |
R-free | 0.24450 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.431 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.850 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rpim | 0.077 | 0.619 |
Number of reflections | 13457 | 1567 |
<I/σ(I)> | 5.5 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 22.2 | 22.3 |
CC(1/2) | 0.993 | 0.181 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | PEG 5K MME, Sodium chloride, Tricine |