7RD5
Crystal structure of Tspan15 large extracellular loop (Tspan15 LEL) in complex with 1C12 Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-22 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 171.300, 124.640, 116.120 |
Unit cell angles | 90.00, 98.17, 90.00 |
Refinement procedure
Resolution | 47.500 - 3.600 |
R-factor | 0.2735 |
Rwork | 0.271 |
R-free | 0.30930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6bdz |
RMSD bond length | 0.003 |
RMSD bond angle | 0.736 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.1_4122+SVN) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.500 | 3.729 |
High resolution limit [Å] | 3.600 | 3.600 |
Rmerge | 0.158 | 1.080 |
Rmeas | 0.199 | 1.364 |
Rpim | 0.120 | 0.822 |
Number of reflections | 27150 | 6908 |
<I/σ(I)> | 4.63 | 0.86 |
Completeness [%] | 95.8 | 97.17 |
Redundancy | 2.5 | 2.5 |
CC(1/2) | 0.995 | 0.520 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.1 M sodium acetate pH 4.6, 3.4 M sodium nitrate |